|
KMID : 0380019930080050478
|
|
Korean Journal of Biotechnology and Bioengineering
1993 Volume.8 No. 5 p.478 ~ p.482
|
|
|
High Density Culture of KA112 Hybridoma and Effect of Glucose Concentration on Mab Producitivity
|
|
|
|
|
Abstract
|
|
|
|
Perfusion culture was conducted in Celligen perfusion culture system using a self-constructed hybridoma cell and low serum medium. The culture system employed hollow fiber to separate cells from the culture broth. Maximum cell density of 2.1 x 10´cells/ml, 10 times higher than in batch culture, could be achived. Concentration of monoclonal antibody(MAb) was 4 times higher and production rate at maximum feed rate was 9 times higher than in batch culture. Glucose concentration was very important for the cell growth and MAb production. When glucose concentration was below lg/ Q , i. e. 0.50.9 g / P , specific MAb production rate decreased but cell concentration still increased. As the glucose concentration goes above 1 g / Q , specific MAb production rate increased and remained at maximum value at more than 1.5 g glucose/ P . The maximum value of the specific Mab production rate was similar to that of batch culture.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|